nuclear marker hoescht  (Vector Laboratories)

Journal: Neurogenetics

Article Title: Nramp1 is expressed in neurones and is associated with behavioural and immune responses to stress

doi:

Figure Lengend Snippet: Indirect fluorescent immunochemical triple co-staining of frozen mouse brain sections in a and d and cultured embryonic mouse brain cells in b, c, e and f, all viewed at x400 magnification. Nuclei are stained with Hoescht and appear blue. In (a), (b) and (c), green fluorescence identifies vesicular cytoplasmic Nramp1 expression within neurones that are identified by their morphology and red fluorescence for neuronal marker antibodies NeuN in a and β-tubulin III in b and c. In (d), Nramp1 expression (red) is also seen in macrophages/microglia labelled (green) for a pan-macrophage marker (F4/80). In (e), green fluorescence identifies vesicular cytoplasmic Nramp1 expression within a cultured embryonic microglial cell identified by its morphology and red fluorescence for F4/80. In (f), Nramp1 expression (green) is not seen in GFAP (red) immuno-reactive astrocytes. Polyclonal rabbit anti-Nramp1 antibodies were used in a, b, d and e; monoclonal rat anti-Nramp1 antibodies in c and f. The bar in f represents 10 μm.

Article Snippet: The secondary antibodies used had been raised against IgG from the species of the primary antibodies used in that particular experiment and were both diluted 1:50 in 3% BSA in TTBS together with 1 ng/ml Hoescht (Sigma) nuclear marker.

Techniques: Staining, Cell Culture, Fluorescence, Expressing, Marker